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| Registros recuperados : 19 | |
| 2. |  | ENCINAS, V. B.; SOUZA, W. R. de; TENENTE, R. C. V.; TENENTE, G. C. M. V. Base de dados de nematóides quarentenários para atender à agricultura brasileira. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 6., 2001, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2001. p. 104.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | MONTEIRO, L. M. R.; VOLPATO, M. M. L.; INÁCIO, F. D.; SOUZA, W. R. de; SILVA, T. H.; ALVES, H. M. R. Descrição do clima na região da Indicação Geográfica Campo das Vertentes nos anos de 2020 a 2022. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA, 18., 2023, Belo Horizonte. Anais... Belo Horizonte: EPAMIG, 2023. p. 79-78| Biblioteca(s): Embrapa Café. |
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| 4. | | SANTIAGO, T. R.; DUARTE, K. E.; SOUZA, W. R. de; STEINDORFF, A. S.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Divergence of DNA methylation and gene expression in Setaria viridis accessions under drought stress. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 31.| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | MENEZES, C. B. A. de; AFONSO, R. S.; SOUZA, W. R. de; PARMA, M. M.; MELO, I. S. de; ZUCCHI, T. D.; FANTINATTI-GARBOGGINI, F. Gordonia didemni sp. nov. an actinomycete isolated from the marine ascidium Didemnum sp. Antonie van Leeuwenhoek, Gedrukt, v. 109, n. 2, p. 297-303, 2016.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 6. | | MARTINS, P. K.; MAFRA, V.; SOUZA, W. R. de; RIBEIRO, A. P.; VINECKY, F.; BASSO, M. F.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis. Scientific Reports, V. 6, 28348, 2016.| Biblioteca(s): Embrapa Agroenergia. |
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| 7. | | MENEZES, C. B. A. de; AFONSO, R. S.; SOUZA, W. R. de; PARMA, M. M.; MELO, I. S. de; ZUCCHI, T. D.; FANTINATTI-GARBOGGINI, F. Williamsia spongiae sp. nov., an actinomycete isolated from the marine sponge Amphimedon viridis. International Journal of Systematic and Evolutionary Microbiology, v. 67, n. 5, p. 1260-1265, 2017.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | DUARTE, K. E.; SOUZA, W. R. de; SANTIAGO, T. S.; SAMAPAIO, B. L.; RIBEIRO, A. P.; COTTA, M. G.; DIAS, B. B. A.; MARRACCINI, P. R. R.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Identification and characterization of core abscisic acid (ABA) signaling components and their gene expression profile in response to abiotic stresses in Setaria viridis. Scientific Reports, v. 9, n . 4028, 2019. 16 p.| Biblioteca(s): Embrapa Agroenergia. |
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| 9. | | MARTINS, M. T. B.; SOUZA, W. R. de; DIAS, B. B. A.; BASSO, M. F.; OLIVEIRA, N. G. de; VINECKY, F.; MARTINS, P. K.; OLIVEIRA, P. A. de; ARENQUE-MUSA, B. C.; SOUZA, A. P. de; BUCKERIDGE, M. S.; KOBAYASHI, A. K.; QUIRINO, B. F.; MOLINARI, H. B. C. Characterization of sugarcane (Saccharum spp.) leaf senescence: implications for biofuel production. Biotechnology for Biofuels, v. 9, n. 153, 2016.| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | FIDELIS, R. R.; SERAGLIO, N. A.; RAUBER, W. A.; MARQUES, K. R.; BORTOLON, L.; BORTOLON, E. S. O.; TAVARES, A. T.; ROTILI, E. A.; FREITAS, G. A. de; SOUZA, W. R. de; BARROS, H. B.; LEAL, T. H. V. L.; SOUSA, P. L. R.; BURIN, L. X. Kinetin and nitrogen in agronomic characteristics of soybean. American Scientific Research Journal for Engineering, Technology, and Sciences, v. 69, n. 1, p. 113-120, 2020.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 11. | | SOUZA, W. R. de; OLIVEIRA, N. G. de; VINECKY, F.; RIBEIRO, A. P.; BASSO, M. F.; CASARI, R. A. das C. N.; DIAS, B. B. A.; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; MOLINARI, H. B. C. Development of drought-tolerant sugarcane overexpressing the AtDREB2A CA gene. In: NAKASHIMA, K.; URAO, Takeshi. (Ed.). Development of biotechnologies and biotech crops for stable food production under adverse environments and changing climate conditions. Tsukuba: JIRCAS, 2020. ch. 3-3. p. 89-98 (JIRCAS Working Report, 91).| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | RIBEIRO, A. P.; VINECKY, F.; DUARTE, K. E.; SANTIAGO, T. R.; CASARI, R. A. das C. N.; HELL, A. F.; DIAS, B. B. A.; MARTINS, P. K.; CENTENO, D. da C.; OLIVEIRA, P. A. de; CANÇADO, G. M. de A.; MAGALHAES, J. V. de; KOBAYASHI, A. K.; SOUZA, W. R. de; MOLINARI, H. B. C. Enhanced aluminum tolerance in sugarcane: evaluation of SbMATE overexpression and genome-wide identification of ALMTs in Saccharum spp. BMC Plant Biology, v. 21, p. 1-15, 2021. Article number: 300.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Milho e Sorgo. |
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| 13. | | DUARTE, K E. D.; BASSO, M. F.; OLIVEIRA, N. G. de; SILVA, J. C. F. da; GARCIA, B. de O.; DIAS, B. B. A.; CARDOSO, T. B.; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; SANTIAGO, T. R.; SOUZA, W. R. de; MOLINARI, H. B. C. MicroRNAs expression profiles in early responses to different levels of water deficit in Setaria viridis. Physiology and Molecular Biology of Plants, v. 28, n. 8, p. 1607-1624, 2022.| Biblioteca(s): Embrapa Agroenergia; Embrapa Soja. |
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| 14. | | RIBEIRO, A. P.; SOUZA, W. R. de; MARTINS, P. K.; VINECKY, F.; DUARTE, K. E.; BASSO, M. F.; CUNHA, B. A. D. B. da; CAMPANHA, R. B.; OLIVEIRA, P. A. de; CENTENO, D. C.; CANÇADO, G. M. A.; MAGALHÃES, J. V. de; SOUSA, C. A. F. de; ANDRADE, A. C.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Overexpression of BdMATE Gene improves aluminum tolerance in Setaria viridis. Frontiers in Plant Science, v. 8, artigo 685, 2017.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | RIBEIRO, A. P.; SOUZA, W. R. de; MARTINS, P. K.; VINECKY, F.; DUARTE, K. E.; BASSO, M. F.; DIAS, B. B. A.; CAMPANHA, R. B.; OLIVEIRA, P. A. de; CENTENO, D. C.; CANÇADO, G. M. de A.; MAGALHÃES, J. V. de; SOUSA, C. A. F. de; ANDRADE, A. C.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Overexpression of BdMATE gene improves aluminum tolerance in Setaria viridis. Frontiers in Plant Science, v. 8, p. 1-12, June 2017. 12 p.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
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| 16. | | SOUZA, W. R. de; PACHECO, T. F.; DUARTE, K. E.; SAMPAIO, B. L.; OLIVEIRA, P. A. de; MARTINS, P. M.; SANTIAGO, T. R.; FORMIGHIERI, E. F.; VINECKY, F.; RIBEIRO, A. P.; DIAS, B. B. A.; KOBAYASHI, A. K.; MITCHELL, R. A. C.; RODRIGUES, D. de S.; MOLINARI, H. B. C. Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019.| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | MOTA, T. R.; SOUZA, W. R. DE; OLIVEIRA, D. M.; MARTINS, P. K.; SAMPAIO, B. L.; VINECKY, F.; RIBEIRO, ANA. P.; DUARTE, K. E.; PACHECO, T. F.; MONTEIRO, N. DE K. V.; CAMPANHA, R. B.; MARCHIOSI, R.; VIEIRA, D. S.; KOBAYASHI, A. K.; OLIVEIRA, P. A. de; FERRARESE-FILHO, O.; MITCHELL, R. A. C.; MOLINARI, H. B. C.; SANTOS, W. D. D. Suppression of a BAHD acyltransferase decreases p-coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis. The Plant Journal, n. 105, p. 136-150, 2021.| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | SOUZA, W. R. de; OLIVEIRA, N. G. de; VINECY, F.; RIBEIRO, A. P.; BASSO, M. F.; CASARI, R. A. das C. N.; CUNHA, B. A. D. B. da; DUARTE, K. E.; SANTIAGO, T. R.; MARTIN, P. K.; AUCIQUE-PEREZ, C. E.; CRISTOFOLETTI JUNIOR, S. C.; NEPOMUCENO, A. L.; SOUSA, C. A. F. de; KOBAYASHI, A. K.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; MOLINARI, H. B. C. Field evaluation of AtDREB2A CA overexpressing sugarcane for drought tolerance. Journal Agronomy and Crop Science, v. 00, p. 1-9, Apr. 2019.| Biblioteca(s): Embrapa Agroenergia; Embrapa Meio-Norte. |
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| 19. | | SOUZA, W. R. de; MARTINS, P. K; FREEMAN, J.; PELLNY, T. K.; MICHAELSON, L. V.; SAMPAIO, B. L.; VINECKY, F.; RIBEIRO, A. P.; DIAS, B. B. A.; KOBAYASHI, A. K.; OLIVEIRA, P. A. de; CAMPANHA, R. B.; PACHECO, T. F.; MARTARELLO, D. C. I.; MARCHIOSI, R.; FERRARESE-FILHO, O.; SANTOS, W. D. dos; TRAMONTINA, R.; SQUINA, F. M.; CENTENO, D. C.; GASPAR, M.; BRAGA, M. R.; TINÉ, M. A. S.; RALPH, J.; MITCHELL, R. A. C.; MOLINARI, H. B. C. Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility. New Phytologist, v. 218, p. 81-93, 2018.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 19 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
21/06/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUZA, W. R. de; PACHECO, T. F.; DUARTE, K. E.; SAMPAIO, B. L.; OLIVEIRA, P. A. de; MARTINS, P. M.; SANTIAGO, T. R.; FORMIGHIERI, E. F.; VINECKY, F.; RIBEIRO, A. P.; DIAS, B. B. A.; KOBAYASHI, A. K.; MITCHELL, R. A. C.; RODRIGUES, D. de S.; MOLINARI, H. B. C. |
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Afiliação: |
Wagner Rodrigo de Souza, Bolsista da Embrapa Agroenergia; THALYTA FRAGA PACHECO, CNPAE; Karoline Estefani Duarte, Bolsista da Embrapa Agroenergia; Bruno Leite Sampaio, Bolsista da Embrapa Agroenergia; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; Polyana Kelly Martins, Bolsista da Embrapa Agroenergia; Thaís Ribeiro Santiago, Bolsista da Embrapa Agroenergia; EDUARDO FERNANDES FORMIGHIERI, CNPAE; Felipe Vinecky; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; Rowan Andrew Craig Mitchell; DASCIANA DE SOUSA RODRIGUES, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane, MenosBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol productio... Mostrar Tudo |
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Palavras-Chave: |
Cell-wall acylation; Lignocellulosic feedstock. |
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Thesaurus NAL: |
Biofuels; Biomass; Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198722/1/Silencing-of-a-BAHD-acyltransferase.pdf
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Marc: |
LEADER 02739naa a2200349 a 4500
001 2109978
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aSOUZA, W. R. de
245 $aSilencing of a BAHD acyltransferase in sugarcane increases biomass digestibility.$h[electronic resource]
260 $c2019
520 $aBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane,
650 $aBiofuels
650 $aBiomass
650 $aSugarcane
653 $aCell-wall acylation
653 $aLignocellulosic feedstock
700 1 $aPACHECO, T. F.
700 1 $aDUARTE, K. E.
700 1 $aSAMPAIO, B. L.
700 1 $aOLIVEIRA, P. A. de
700 1 $aMARTINS, P. M.
700 1 $aSANTIAGO, T. R.
700 1 $aFORMIGHIERI, E. F.
700 1 $aVINECKY, F.
700 1 $aRIBEIRO, A. P.
700 1 $aDIAS, B. B. A.
700 1 $aKOBAYASHI, A. K.
700 1 $aMITCHELL, R. A. C.
700 1 $aRODRIGUES, D. de S.
700 1 $aMOLINARI, H. B. C.
773 $tBiotechnology for Biofuels$gv. 12, n. 111, p. 1-14, 2019.
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Embrapa Agroenergia (CNPAE) |
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